Review



mouse anti mf20  (Developmental Studies Hybridoma Bank)


Bioz Verified Symbol Developmental Studies Hybridoma Bank is a verified supplier
Bioz Manufacturer Symbol Developmental Studies Hybridoma Bank manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 95
      Buy from Supplier

    Structured Review

    Developmental Studies Hybridoma Bank mouse anti mf20
    Mouse Anti Mf20, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+mf20/anti-Myosin+heavy+chain/pm41601327-122-31-33
    Average 95 stars, based on 18 article reviews
    mouse anti mf20 - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Purification:

    Article Title: Neural crest cell-derived DKK1 and NEDD4 modulate Wnt signalling in the second heart field to orchestrate outflow tract development
    Article Snippet: For immunolabelling, cryosections, whole embryos, or fixed cells were blocked in 10% DAKO block, 0.2% BSA, 0.2% Triton X-100 in PBS, and stained with the indicated primary antibodies. .. Antibodies used were rabbit anti-NEDD4 (Abcam 14592) 1:300; rabbit anti-NEDD4 (purified serum, gift from S. Kumar) 1:300; mouse anti-alpha smooth muscle actin (Sigma A2547) 1:2000; rat anti-CD31 (Biolegend 102502) 1:150; goat anti-SOX10 (R&D Systems AF2864) 1:200; chicken anti-GFP (Abcam ab13970) 1:1000; mouse anti-Isl1 (DSHB 39.4D5 or 40.3A4) 1:50; mouse anti-MF20 (DSHB) 1:100; goat anti-DKK1 (R&D Systems AF1765) 1:200; mouse anti-AP2a (DSHB 3B5) 1:20; rabbit anti-beta-catenin non-pS45 (Cell Signaling Technology 19807) 1:200; mouse anti-beta-catenin ABC (8E7) (Millipore 05-665) 1:100; mouse anti-beta-catenin pY489 (DSHB) 1:50; mouse anti-FLAG (Sigma F3165) 1:1000; mouse anti-GM130 (BD 610822) 1:100; rabbit anti-phospho-Histone H3 (Millipore 06-570) 1:500; rabbit anti-cleaved-Caspase-3 (Cell Signaling Technology 9661) 1:500; rabbit anti-phospho-SMAD1/5/9 (Cell Signaling Technology 13820) 1:200; rabbit anti-phospho-ERK1/2 (Cell Signaling Technology 4370) 1:100; goat anti-Scribble (Santa Cruz Biotechnology sc-11048) 1:50; rabbit anti-Laminin (Sigma L9393) 1:1000; rabbit anti-Fibronectin (DakoCytomation A0245) 1:1000; mouse anti-N-cadherin (Cell Signaling Technology 14215) 1:100; AlexaFluor 647 conjugated Phalloidin (Invitrogen) 1:200. .. EdU staining was performed following manufacturers recommendations (Invitrogen Click-iT EdU AlexaFluor 555 Cell Proliferation Kit).

    Article Title: Neural crest cell-derived DKK1 and NEDD4 modulate Wnt signalling in the second heart field to orchestrate outflow tract development.
    Article Snippet: For immunolabelling, cryosections, whole embryos or fixed cells were blocked in 10% DAKO block, 0.2% BSA, 0.2% Triton X-100 in PBS, and stained with the indicated primary antibodies. .. Antibodies used were rabbit anti-NEDD4 (Abcam 14592) 1:300; rabbit anti-NEDD4 (purified serum, gift from S. Kumar) 1:300; mouse anti-alpha smooth muscle actin (Sigma A2547) 1:2000; rat anti-CD31 (Biolegend 102502) 1:150; goat anti-SOX10 (R&D Systems AF2864) 1:200; chicken anti-GFP (Abcam ab13970) 1:1000; mouse anti-Isl1 (DSHB 39.4D5 or 40.3A4) 1:50; mouse anti-MF20 (DSHB) 1:100; goat anti-DKK1 (R&D Systems AF1765) 1:200; mouse anti-AP2a (DSHB 3B5) 1:20; rabbit anti-beta-catenin non-pS45 (Cell Signaling Technology 19807) 1:200; mouse anti-beta-catenin ABC (8E7) (Millipore 05-665) 1:100; mouse anti-beta-catenin pY489 (DSHB) 1:50; mouse antiFLAG (Sigma F3165) 1:1000; mouse anti-GM130 (BD 610822) 1:100; rabbit antiphospho-Histone H3 (Millipore 06-570) 1:500; rabbit anti-cleaved-Caspase-3 (Cell AR TI CL E IN P RE SS Signaling Technology 9661) 1:500; rabbit anti-phospho-SMAD1/5/9 (Cell Signaling Technology 13820) 1:200; rabbit anti-phospho-ERK1/2 (Cell Signaling Technology 4370) 1:100; goat anti-Scribble (Santa Cruz Biotechnology sc-11048) 1:50; rabbit antiLaminin (Sigma L9393) 1:1000; rabbit anti-Fibronectin (DakoCytomation A0245) 1:1000; mouse anti-N-cadherin (Cell Signaling Technology 14215) 1:100; Alexa Fluor 647 conjugated Phalloidin (Invitrogen) 1:200. .. EdU staining was performed following manufacturers recommendations (Invitrogen Click-iT EdU Alexa Fluor 555 Cell Proliferation Kit).

    Staining:

    Article Title: In situ magnetic-field-assisted bioprinting process using magnetorheological bioink to obtain engineered muscle constructs
    Article Snippet: Then the cells were permeabilized using 2 % Triton X-100 solution and treated with 2 % bovine serum albumin (BSA; Sigma-Aldrich, USA) for 2 h at 37 °C. .. Then, the bioconstructs were treated with mouse anti-piezo1 (5 μg mL −1 in PBS; Invitrogen), mouse anti-MF20 (5 μg mL −1 in PBS; Developmental Studies Hybridoma Bank, USA), rabbit anti-α-actinin (5 μg mL −1 in PBS; PA5-27863; Invitrogen) primary antibodies overnight at 4 °C, followed by staining with Alexa Fluor 488- and 594-conjugated secondary antibodies (1:50 in PBS; Invitrogen) for 1 h at 37 °C. ..

    Incubation:

    Article Title: Crip2 preserves hematopoietic stem and progenitor cell production through inhibition of Notch signals.
    Article Snippet: Briefly, embryos were D ev el o pm en t • A cc ep te d m an us cr ip t dechorionated and fixed in 1% methanol-free formaldehyde (Poly Sciences, 04018) for one hour at room temperature, then rinsed twice in PBS and blocked with 2 mg/ml bovine serum albumin (Sigma Aldrich, A8022), 10% goat serum (Jackson ImmunoResearch, 005000121), and 0.2% saponin (Sigma Aldrich, S4521) in PBS for two hours at room temperature. .. The embryos were incubated overnight at 4C with primary antibodies in 0.2% saponin/PBS solution with the following dilutions: 1:20 mouse anti-S46 (Developmental Studies Hybridoma Bank), 1:500 rabbit anti-dsRed (Clonetech, 632496), 1:20 mouse anti-MF20 (Developmental Studies Hybridoma Bank), or 1:200 rabbit anti-Elnb (Song et al., 2019). .. The next day, embryos were washed twice in 0.2% saponin/PBS solution and incubated for two hours at room temperature with secondary antibodies in 0.2% saponin/PBS with the following dilutions: 1:200 anti-mouse IgG2b AlexaFlour 568 (Invitrogen, A21144), 1:200 anti-rabbit IgG AlexaFlour 488 (Invitrogen, A11034), 1:375 anti-rabbit IgG AlexaFlour 568 (Invitrogen, A11011), or 1:200 anti-mouse IgG1 AlexaFlour 488 (Invitrogen, A21121).

    Immunofluorescence:

    Article Title: A cardiac transcriptional enhancer is repurposed during regeneration to activate an anti-proliferative program.
    Article Snippet: Immunofluorescence – Primary antibodies used in this study were: Rabbit anti-Mef2 (1:100, Abcam, ab197070), Rabbit anti-GFP (1:200, Life Technologies A11122), Mouse anti-MF20 (1:100, Developmental Studies Hybridoma Bank, MF20), and Mouse anti-Collagen I (1:10, Developmental Studies Hybridoma Bank, SP1.D8-s). .. Immunofluorescence – Primary antibodies used in this study were: Rabbit anti-Mef2 (1:100, Abcam, ab197070), Rabbit anti-GFP (1:200, Life Technologies A11122), Mouse anti-MF20 (1:100, Developmental Studies Hybridoma Bank, MF20), and Mouse anti-Collagen I (1:10, Developmental Studies Hybridoma Bank, SP1.D8-s). ..



    Similar Products

    mouse anti mf20  (Developmental Studies Hybridoma Bank)
    95
    Developmental Studies Hybridoma Bank mouse anti mf20
    Mouse Anti Mf20, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+mf20/anti-Myosin+heavy+chain/pm41601327-122-31-33
    Average 95 stars, based on 1 article reviews
    mouse anti mf20 - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    anti myosin heavy chain anti mf20 mouse primary antibody  (Developmental Studies Hybridoma Bank)
    95
    Developmental Studies Hybridoma Bank anti myosin heavy chain anti mf20 mouse primary antibody
    Expression of SARS-CoV-2 spike (S) protein in fibroblasts and muscle models in vitro stimulated with BNT162b2 vaccine. Fibroblasts (A) , myogenic progenitors (MP) (B) , myotubes (MT) (C) and 3D muscle-like tissue (3D-MT) (D) were left not stimulated (NS) or treated with BNT162b2 vaccine (VAX, 1 µg/mL) for 24 h (H) . Immunofluorescence analysis was conducted by confocal microscopy to assess the expression of S protein. Fibroblasts were stained with DAPI (blue) to identify nuclei and with an anti-S antibody (Ab) (green) (A) . For MP staining, in addition to DAPI (blue) and anti-S Ab (green), an anti-phalloidin Ab (red) was used as a marker of undifferentiated muscle cells (B) , while for MT and 3D-MT, staining with <t>anti-myosin</t> <t>heavy</t> <t>chain</t> (MyHC) Ab (red) was employed as a differentiation marker. Representative images, out of three experiments ( n = 3) separately performed, are shown in panels (A–D) . Scale bar: 20 μm.
    Anti Myosin Heavy Chain Anti Mf20 Mouse Primary Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+mf20/anti-Myosin+heavy+chain/pmc12572801-61-40-47
    Average 95 stars, based on 1 article reviews
    anti myosin heavy chain anti mf20 mouse primary antibody - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    mouse monoclonal igg2b anti mf20  (Developmental Studies Hybridoma Bank)
    95
    Developmental Studies Hybridoma Bank mouse monoclonal igg2b anti mf20
    Expression of SARS-CoV-2 spike (S) protein in fibroblasts and muscle models in vitro stimulated with BNT162b2 vaccine. Fibroblasts (A) , myogenic progenitors (MP) (B) , myotubes (MT) (C) and 3D muscle-like tissue (3D-MT) (D) were left not stimulated (NS) or treated with BNT162b2 vaccine (VAX, 1 µg/mL) for 24 h (H) . Immunofluorescence analysis was conducted by confocal microscopy to assess the expression of S protein. Fibroblasts were stained with DAPI (blue) to identify nuclei and with an anti-S antibody (Ab) (green) (A) . For MP staining, in addition to DAPI (blue) and anti-S Ab (green), an anti-phalloidin Ab (red) was used as a marker of undifferentiated muscle cells (B) , while for MT and 3D-MT, staining with <t>anti-myosin</t> <t>heavy</t> <t>chain</t> (MyHC) Ab (red) was employed as a differentiation marker. Representative images, out of three experiments ( n = 3) separately performed, are shown in panels (A–D) . Scale bar: 20 μm.
    Mouse Monoclonal Igg2b Anti Mf20, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+mf20/anti-Myosin+heavy+chain/pmc12355108-6-0-5
    Average 95 stars, based on 1 article reviews
    mouse monoclonal igg2b anti mf20 - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    primary antibody mouse monoclonal igg2b anti mf20 dshb mf 20 if  (Developmental Studies Hybridoma Bank)
    95
    Developmental Studies Hybridoma Bank primary antibody mouse monoclonal igg2b anti mf20 dshb mf 20 if
    Expression of SARS-CoV-2 spike (S) protein in fibroblasts and muscle models in vitro stimulated with BNT162b2 vaccine. Fibroblasts (A) , myogenic progenitors (MP) (B) , myotubes (MT) (C) and 3D muscle-like tissue (3D-MT) (D) were left not stimulated (NS) or treated with BNT162b2 vaccine (VAX, 1 µg/mL) for 24 h (H) . Immunofluorescence analysis was conducted by confocal microscopy to assess the expression of S protein. Fibroblasts were stained with DAPI (blue) to identify nuclei and with an anti-S antibody (Ab) (green) (A) . For MP staining, in addition to DAPI (blue) and anti-S Ab (green), an anti-phalloidin Ab (red) was used as a marker of undifferentiated muscle cells (B) , while for MT and 3D-MT, staining with <t>anti-myosin</t> <t>heavy</t> <t>chain</t> (MyHC) Ab (red) was employed as a differentiation marker. Representative images, out of three experiments ( n = 3) separately performed, are shown in panels (A–D) . Scale bar: 20 μm.
    Primary Antibody Mouse Monoclonal Igg2b Anti Mf20 Dshb Mf 20 If, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+mf20/anti-Myosin+heavy+chain/pmc12355108__mmc1-27-51-57
    Average 95 stars, based on 1 article reviews
    primary antibody mouse monoclonal igg2b anti mf20 dshb mf 20 if - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    anti myhc dshb mf20 mouse  (Developmental Studies Hybridoma Bank)
    95
    Developmental Studies Hybridoma Bank anti myhc dshb mf20 mouse
    Expression of SARS-CoV-2 spike (S) protein in fibroblasts and muscle models in vitro stimulated with BNT162b2 vaccine. Fibroblasts (A) , myogenic progenitors (MP) (B) , myotubes (MT) (C) and 3D muscle-like tissue (3D-MT) (D) were left not stimulated (NS) or treated with BNT162b2 vaccine (VAX, 1 µg/mL) for 24 h (H) . Immunofluorescence analysis was conducted by confocal microscopy to assess the expression of S protein. Fibroblasts were stained with DAPI (blue) to identify nuclei and with an anti-S antibody (Ab) (green) (A) . For MP staining, in addition to DAPI (blue) and anti-S Ab (green), an anti-phalloidin Ab (red) was used as a marker of undifferentiated muscle cells (B) , while for MT and 3D-MT, staining with <t>anti-myosin</t> <t>heavy</t> <t>chain</t> (MyHC) Ab (red) was employed as a differentiation marker. Representative images, out of three experiments ( n = 3) separately performed, are shown in panels (A–D) . Scale bar: 20 μm.
    Anti Myhc Dshb Mf20 Mouse, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+mf20/anti-Myosin+heavy+chain/pmc12510276__ADMA-37-e01147-s002-117-24-25
    Average 95 stars, based on 1 article reviews
    anti myhc dshb mf20 mouse - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    mouse monoclonal anti myh1e mf20 dshb cat ab 2147781  (Developmental Studies Hybridoma Bank)
    96
    Developmental Studies Hybridoma Bank mouse monoclonal anti myh1e mf20 dshb cat ab 2147781
    Expression of SARS-CoV-2 spike (S) protein in fibroblasts and muscle models in vitro stimulated with BNT162b2 vaccine. Fibroblasts (A) , myogenic progenitors (MP) (B) , myotubes (MT) (C) and 3D muscle-like tissue (3D-MT) (D) were left not stimulated (NS) or treated with BNT162b2 vaccine (VAX, 1 µg/mL) for 24 h (H) . Immunofluorescence analysis was conducted by confocal microscopy to assess the expression of S protein. Fibroblasts were stained with DAPI (blue) to identify nuclei and with an anti-S antibody (Ab) (green) (A) . For MP staining, in addition to DAPI (blue) and anti-S Ab (green), an anti-phalloidin Ab (red) was used as a marker of undifferentiated muscle cells (B) , while for MT and 3D-MT, staining with <t>anti-myosin</t> <t>heavy</t> <t>chain</t> (MyHC) Ab (red) was employed as a differentiation marker. Representative images, out of three experiments ( n = 3) separately performed, are shown in panels (A–D) . Scale bar: 20 μm.
    Mouse Monoclonal Anti Myh1e Mf20 Dshb Cat Ab 2147781, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+mf20/anti-Troponin+T/pmc11387278__18_2024_5334_MOESM1_ESM-46-16-20
    Average 96 stars, based on 1 article reviews
    mouse monoclonal anti myh1e mf20 dshb cat ab 2147781 - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    mouse anti-mf20 antibody concentrate  (Developmental Studies Hybridoma Bank)
    90
    Developmental Studies Hybridoma Bank mouse anti-mf20 antibody concentrate
    DCHS1-HA expression at embryonic day 11.5. ( A ) IHC of E11.5 embryos for HA (red) and CD31 (green) showing co-localization of Dchs1-HA and a subset of endothelial cells and expression within the pericardium. Scale bar = 170 μm ( B ) IHC of HA (red) and <t>MF20</t> (green) showing no detectable expression of DCHS1 in cardiomyocytes. Scale bar = 170 μm ( C ) IHC of HA (red) and MF20 (green) in the atrioventricular (AV) cushions showed robust DCHS1 expression as well as expression within the epicardium. Scale bar = 80 μm ( D ) Western analyses of heart lysates at E11.5 probed for HA showing full-length (>250 kDa) and smaller fragments. ( E ) Ponceau-S staining of western blot showing equal loading of proteins. Lumen (Lum), myocardium (Myo), epicardium (Epi), pericardium (Peri), atrioventricular cushions (AVC).
    Mouse Anti Mf20 Antibody Concentrate, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+mf20/mf20+antibody/pmc12153708-59-36-39
    Average 90 stars, based on 1 article reviews
    mouse anti-mf20 antibody concentrate - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Expression of SARS-CoV-2 spike (S) protein in fibroblasts and muscle models in vitro stimulated with BNT162b2 vaccine. Fibroblasts (A) , myogenic progenitors (MP) (B) , myotubes (MT) (C) and 3D muscle-like tissue (3D-MT) (D) were left not stimulated (NS) or treated with BNT162b2 vaccine (VAX, 1 µg/mL) for 24 h (H) . Immunofluorescence analysis was conducted by confocal microscopy to assess the expression of S protein. Fibroblasts were stained with DAPI (blue) to identify nuclei and with an anti-S antibody (Ab) (green) (A) . For MP staining, in addition to DAPI (blue) and anti-S Ab (green), an anti-phalloidin Ab (red) was used as a marker of undifferentiated muscle cells (B) , while for MT and 3D-MT, staining with anti-myosin heavy chain (MyHC) Ab (red) was employed as a differentiation marker. Representative images, out of three experiments ( n = 3) separately performed, are shown in panels (A–D) . Scale bar: 20 μm.

    Journal: Frontiers in Immunology

    Article Title: A biomimetic model composed of injectable 3D muscle-like tissue, stromal and immune cells for recapitulating the rapid immune signature predictive of mRNA vaccine immunogenicity

    doi: 10.3389/fimmu.2025.1651095

    Figure Lengend Snippet: Expression of SARS-CoV-2 spike (S) protein in fibroblasts and muscle models in vitro stimulated with BNT162b2 vaccine. Fibroblasts (A) , myogenic progenitors (MP) (B) , myotubes (MT) (C) and 3D muscle-like tissue (3D-MT) (D) were left not stimulated (NS) or treated with BNT162b2 vaccine (VAX, 1 µg/mL) for 24 h (H) . Immunofluorescence analysis was conducted by confocal microscopy to assess the expression of S protein. Fibroblasts were stained with DAPI (blue) to identify nuclei and with an anti-S antibody (Ab) (green) (A) . For MP staining, in addition to DAPI (blue) and anti-S Ab (green), an anti-phalloidin Ab (red) was used as a marker of undifferentiated muscle cells (B) , while for MT and 3D-MT, staining with anti-myosin heavy chain (MyHC) Ab (red) was employed as a differentiation marker. Representative images, out of three experiments ( n = 3) separately performed, are shown in panels (A–D) . Scale bar: 20 μm.

    Article Snippet: Afterward, 2D MP were incubated for 1 h at RT with Phalloidin Alexa Fluor 555 (Invitrogen, Thermo Fisher Scientific, A12379) for labeling F-actin of cytoskeleton, while MT and 3D-MT were incubated for 1 h and o.n. at RT, respectively, with anti-myosin heavy chain (anti-MF20) mouse primary antibody (DSHB, AB_2147781) diluted 1:200 to assess terminal myogenic differentiation.

    Techniques: Expressing, In Vitro, Immunofluorescence, Confocal Microscopy, Staining, Marker

    DCHS1-HA expression at embryonic day 11.5. ( A ) IHC of E11.5 embryos for HA (red) and CD31 (green) showing co-localization of Dchs1-HA and a subset of endothelial cells and expression within the pericardium. Scale bar = 170 μm ( B ) IHC of HA (red) and MF20 (green) showing no detectable expression of DCHS1 in cardiomyocytes. Scale bar = 170 μm ( C ) IHC of HA (red) and MF20 (green) in the atrioventricular (AV) cushions showed robust DCHS1 expression as well as expression within the epicardium. Scale bar = 80 μm ( D ) Western analyses of heart lysates at E11.5 probed for HA showing full-length (>250 kDa) and smaller fragments. ( E ) Ponceau-S staining of western blot showing equal loading of proteins. Lumen (Lum), myocardium (Myo), epicardium (Epi), pericardium (Peri), atrioventricular cushions (AVC).

    Journal: Cells

    Article Title: Dynamic Expression and Functional Implications of the Cell Polarity Gene, Dchs1, During Cardiac Development

    doi: 10.3390/cells14110774

    Figure Lengend Snippet: DCHS1-HA expression at embryonic day 11.5. ( A ) IHC of E11.5 embryos for HA (red) and CD31 (green) showing co-localization of Dchs1-HA and a subset of endothelial cells and expression within the pericardium. Scale bar = 170 μm ( B ) IHC of HA (red) and MF20 (green) showing no detectable expression of DCHS1 in cardiomyocytes. Scale bar = 170 μm ( C ) IHC of HA (red) and MF20 (green) in the atrioventricular (AV) cushions showed robust DCHS1 expression as well as expression within the epicardium. Scale bar = 80 μm ( D ) Western analyses of heart lysates at E11.5 probed for HA showing full-length (>250 kDa) and smaller fragments. ( E ) Ponceau-S staining of western blot showing equal loading of proteins. Lumen (Lum), myocardium (Myo), epicardium (Epi), pericardium (Peri), atrioventricular cushions (AVC).

    Article Snippet: The following primary antibodies and reagents were used for immunostaining: rabbit anti-HA antibody (Cell Signaling, # 3724; Danvers, MN, USA) at a dilution of 1:250, rat anti-CD31 antibody (Dianova, # DIA-310; Hamburg, Germany) at 1:100, and mouse anti-MF20 antibody (Developmental Studies Hybridoma Bank, Concentrate 0.1 mL; Iowa City, IA, USA) at 1:500.

    Techniques: Expressing, Western Blot, Staining

    DCHS1-HA expression at embryonic day 13.5. ( A ) Left ventricular free wall cardiac tissue was co-stained with HA (red) and CD31 (green), showing significant overlap (yellow), demonstrating co-expression within endothelial cells. ( B ) No overlap in staining was detected between MF20 (green) and DCHS1 (red). ( C ) Atrioventricular (AV) cushions demonstrated a gradient of DCHS1 expression, with the highest levels within the subendocardium (**) and lower levels closer to the AV myocardium (*). ( D ) Interventricular septum showed relatively low levels of endocardial DCHS1 expression at this time point. ( E ) Western analyses of heart lysates at E13.5 probed for HA showing full-length (>250 kDa) and smaller fragments. ( F ) Ponceau-S staining of western blot showing equal loading of proteins. Lumen (Lum), trabeculated myocardium (T), myocardium (Myo), epicardium (Epi), and atrioventricular cushions (AVC).

    Journal: Cells

    Article Title: Dynamic Expression and Functional Implications of the Cell Polarity Gene, Dchs1, During Cardiac Development

    doi: 10.3390/cells14110774

    Figure Lengend Snippet: DCHS1-HA expression at embryonic day 13.5. ( A ) Left ventricular free wall cardiac tissue was co-stained with HA (red) and CD31 (green), showing significant overlap (yellow), demonstrating co-expression within endothelial cells. ( B ) No overlap in staining was detected between MF20 (green) and DCHS1 (red). ( C ) Atrioventricular (AV) cushions demonstrated a gradient of DCHS1 expression, with the highest levels within the subendocardium (**) and lower levels closer to the AV myocardium (*). ( D ) Interventricular septum showed relatively low levels of endocardial DCHS1 expression at this time point. ( E ) Western analyses of heart lysates at E13.5 probed for HA showing full-length (>250 kDa) and smaller fragments. ( F ) Ponceau-S staining of western blot showing equal loading of proteins. Lumen (Lum), trabeculated myocardium (T), myocardium (Myo), epicardium (Epi), and atrioventricular cushions (AVC).

    Article Snippet: The following primary antibodies and reagents were used for immunostaining: rabbit anti-HA antibody (Cell Signaling, # 3724; Danvers, MN, USA) at a dilution of 1:250, rat anti-CD31 antibody (Dianova, # DIA-310; Hamburg, Germany) at 1:100, and mouse anti-MF20 antibody (Developmental Studies Hybridoma Bank, Concentrate 0.1 mL; Iowa City, IA, USA) at 1:500.

    Techniques: Expressing, Staining, Western Blot

    DCHS1-HA expression during fetal gestation. ( A ) Left ventricular free wall cardiac tissue was co-stained with HA (red) and CD31 (green), demonstrating significant overlap. At this timepoint, some non-endocardial, non-MF20 (green) staining is evident (arrows). Epicardial expression of DCHS1-HA is prominent (arrowheads). ( B ) HA (red) and MF20 (green) in the LV free wall showing no detectable overlap in expression. ( C ) IHC for HA (red) and MF20 (green) showing a gradient expression of DCHS1-HA, with the highest levels within the mitral valve subendocardium (**) and lower levels closer to the myocardium (*). ( D ) tissue from the interventricular septum was co-stained with HA (for DCHS1-HA) in red and CD31 (endothelial cell marker) in green. ( E ) Whole-heart tissue lysates of pups from an E18.5 litter were probed blindly for HA (to visualize DCHS1-HA), with observed bands highlighted with arrowheads. Arrow represents non-specific (n.s.) antibody staining on the blot. ( F ) Ponceau-S total protein staining of western blot analyzed in ( E ). Labeled abbreviations: lumen (Lum), right ventricular lumen (RV Lum), trabeculated myocardium (T), myocardium (Myo), epicardium (Epi), and pericardium (Peri).

    Journal: Cells

    Article Title: Dynamic Expression and Functional Implications of the Cell Polarity Gene, Dchs1, During Cardiac Development

    doi: 10.3390/cells14110774

    Figure Lengend Snippet: DCHS1-HA expression during fetal gestation. ( A ) Left ventricular free wall cardiac tissue was co-stained with HA (red) and CD31 (green), demonstrating significant overlap. At this timepoint, some non-endocardial, non-MF20 (green) staining is evident (arrows). Epicardial expression of DCHS1-HA is prominent (arrowheads). ( B ) HA (red) and MF20 (green) in the LV free wall showing no detectable overlap in expression. ( C ) IHC for HA (red) and MF20 (green) showing a gradient expression of DCHS1-HA, with the highest levels within the mitral valve subendocardium (**) and lower levels closer to the myocardium (*). ( D ) tissue from the interventricular septum was co-stained with HA (for DCHS1-HA) in red and CD31 (endothelial cell marker) in green. ( E ) Whole-heart tissue lysates of pups from an E18.5 litter were probed blindly for HA (to visualize DCHS1-HA), with observed bands highlighted with arrowheads. Arrow represents non-specific (n.s.) antibody staining on the blot. ( F ) Ponceau-S total protein staining of western blot analyzed in ( E ). Labeled abbreviations: lumen (Lum), right ventricular lumen (RV Lum), trabeculated myocardium (T), myocardium (Myo), epicardium (Epi), and pericardium (Peri).

    Article Snippet: The following primary antibodies and reagents were used for immunostaining: rabbit anti-HA antibody (Cell Signaling, # 3724; Danvers, MN, USA) at a dilution of 1:250, rat anti-CD31 antibody (Dianova, # DIA-310; Hamburg, Germany) at 1:100, and mouse anti-MF20 antibody (Developmental Studies Hybridoma Bank, Concentrate 0.1 mL; Iowa City, IA, USA) at 1:500.

    Techniques: Expressing, Staining, Marker, Western Blot, Labeling

    Dchs1 Expression at post-natal day 0 (P0). ( A ) IHC of left ventricular free wall was performed for HA (red) and CD31 (green) or ( B ) MF20 (green). Consistent with earlier timepoints, DCHS1 was not observed on cardiomyocytes but was detected on endothelial cells. Robust and extensive staining was also observed throughout the myocardium in cells that are negative for CD31 and MF20 (indicated by arrows in 1A), likely representing a fibroblast population. ( C ) IHC in mitral valve displayed extensive staining throughout the leaflet, with the most abundant expression within the subendocardial mesenchyme. ( D ) IHC in the interventricular septum was similar with the LV free wall in that DCHS1 is co-expressed with CD31 as well as prevalent in a non-myocyte, non-endothelial cell population. ( E ) Western analyses of whole-heart lysates revealed maintenance of the full-length protein and presence of a small-molecular-weight fragment (arrowheads). Arrow represents non-specific (n.s.) antibody staining on the blot. ( F ) Ponceau-S total protein staining demonstrates equal loading between samples. Lumen (Lum), right/left ventricular lumen (RV/LV, respectively, lumen), trabeculated myocardium (T), myocardium (Myo), epicardium (Epi), mitral valve (MV).

    Journal: Cells

    Article Title: Dynamic Expression and Functional Implications of the Cell Polarity Gene, Dchs1, During Cardiac Development

    doi: 10.3390/cells14110774

    Figure Lengend Snippet: Dchs1 Expression at post-natal day 0 (P0). ( A ) IHC of left ventricular free wall was performed for HA (red) and CD31 (green) or ( B ) MF20 (green). Consistent with earlier timepoints, DCHS1 was not observed on cardiomyocytes but was detected on endothelial cells. Robust and extensive staining was also observed throughout the myocardium in cells that are negative for CD31 and MF20 (indicated by arrows in 1A), likely representing a fibroblast population. ( C ) IHC in mitral valve displayed extensive staining throughout the leaflet, with the most abundant expression within the subendocardial mesenchyme. ( D ) IHC in the interventricular septum was similar with the LV free wall in that DCHS1 is co-expressed with CD31 as well as prevalent in a non-myocyte, non-endothelial cell population. ( E ) Western analyses of whole-heart lysates revealed maintenance of the full-length protein and presence of a small-molecular-weight fragment (arrowheads). Arrow represents non-specific (n.s.) antibody staining on the blot. ( F ) Ponceau-S total protein staining demonstrates equal loading between samples. Lumen (Lum), right/left ventricular lumen (RV/LV, respectively, lumen), trabeculated myocardium (T), myocardium (Myo), epicardium (Epi), mitral valve (MV).

    Article Snippet: The following primary antibodies and reagents were used for immunostaining: rabbit anti-HA antibody (Cell Signaling, # 3724; Danvers, MN, USA) at a dilution of 1:250, rat anti-CD31 antibody (Dianova, # DIA-310; Hamburg, Germany) at 1:100, and mouse anti-MF20 antibody (Developmental Studies Hybridoma Bank, Concentrate 0.1 mL; Iowa City, IA, USA) at 1:500.

    Techniques: Expressing, Staining, Western Blot, Molecular Weight

    Adolescent and adult Dchs1 expression. ( A , B ) Post-natal LV free wall and mitral valves at day 7 (P7) and P21 mice were stained with HA (red) and MF20 (green). ( C , D ) Western analyses from whole-heart tissue lysates of pups from P7 and P21 probed for HA. Full length (>250 kDa) was barely detectable at this stage, and reduction in the smaller molecular weight is also apparent at both P7 and P21 (arrowheads). At these timepoints, additional smaller fragments are observed, indicating potential degradation of DCHS1. Arrow represents non-specific (n.s.) immunoreactive bands. ( E , F ) Ponceau-S stain showing equivalent protein loading, respectively. Labeled abbreviations: lumen (Lum), trabeculated myocardium (T), myocardium (Myo), epicardium (Epi), mitral valve (MV), and interventricular septum (IVS).

    Journal: Cells

    Article Title: Dynamic Expression and Functional Implications of the Cell Polarity Gene, Dchs1, During Cardiac Development

    doi: 10.3390/cells14110774

    Figure Lengend Snippet: Adolescent and adult Dchs1 expression. ( A , B ) Post-natal LV free wall and mitral valves at day 7 (P7) and P21 mice were stained with HA (red) and MF20 (green). ( C , D ) Western analyses from whole-heart tissue lysates of pups from P7 and P21 probed for HA. Full length (>250 kDa) was barely detectable at this stage, and reduction in the smaller molecular weight is also apparent at both P7 and P21 (arrowheads). At these timepoints, additional smaller fragments are observed, indicating potential degradation of DCHS1. Arrow represents non-specific (n.s.) immunoreactive bands. ( E , F ) Ponceau-S stain showing equivalent protein loading, respectively. Labeled abbreviations: lumen (Lum), trabeculated myocardium (T), myocardium (Myo), epicardium (Epi), mitral valve (MV), and interventricular septum (IVS).

    Article Snippet: The following primary antibodies and reagents were used for immunostaining: rabbit anti-HA antibody (Cell Signaling, # 3724; Danvers, MN, USA) at a dilution of 1:250, rat anti-CD31 antibody (Dianova, # DIA-310; Hamburg, Germany) at 1:100, and mouse anti-MF20 antibody (Developmental Studies Hybridoma Bank, Concentrate 0.1 mL; Iowa City, IA, USA) at 1:500.

    Techniques: Expressing, Staining, Western Blot, Molecular Weight, Labeling